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Comparison of standard surface chemistries for culturing mesenchymal stem cells prior to neural differentiation

Paper ID Volume ID Publish Year Pages File Format Full-Text
11974 770 2006 7 PDF Available
Title
Comparison of standard surface chemistries for culturing mesenchymal stem cells prior to neural differentiation
Abstract

A critical element of any stem cell differentiation protocol is the ability to compare its effects relative to an undifferentiated population of the same cells. In an attempt to standardize pre-differentiation conditions of adult derived mesenchymal stem cells prior to neural induction experiments, we asked what is the simplest chemical surface that supports the growth and maintenance of these cells in a pre-differentiation state. Adult bone marrow-derived rat mesenchymal stem cells (BMSCs) were expanded in vitro on Permanox Lab-Tek® tissue culture treated plastic (TCP), poly-d-lysine (PDL) coated glass, PDL-laminin-1 coated glass, and untreated glass. TCP provided the best surface for maintaining morphologies generally considered to be undifferentiated, while PDL coated glass and uncoated glass provided the least suitable surfaces. Expansion of BMSCs on PDL-laminin-1 coated glass resulted in expression of nestin, a marker associated with neuronal and other progenitor cells, and therefore may confound experimental results if used as a pre-differentiation surface.

Keywords
Stem cells; Cell culture; Surface treatment; Neural cells
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Comparison of standard surface chemistries for culturing mesenchymal stem cells prior to neural differentiation
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 27, Issue 24, August 2006, Pages 4333–4339
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us