fulltext.study @t Gmail

The effect of charged groups on protein interactions with poly(HEMA) hydrogels

Paper ID Volume ID Publish Year Pages File Format Full-Text
11985 771 2006 9 PDF Available
Title
The effect of charged groups on protein interactions with poly(HEMA) hydrogels
Abstract

Proteins, lipids and other biomolecules interact strongly with the acrylic-based biomaterials used for contact lenses. Although hydrogels are nominally resistant to protein fouling, many studies have reported considerable amounts of protein bound to poly(2-hydroxyethylmethacrylate) (PHEMA) lenses. This study examined the binding of a series of biomolecules (tear protein analogues, mucin and cholesterol) to poly(methylmethacrylate) (PMMA) and three HEMA-based hydrogels (PHEMA, HEMA plus methacrylic acid (P(HEMA–MAA)), HEMA plus methacrylic acid plus N-vinylpyrrolidone (P(HEMA–MAA–NVP))) by use of a quartz crystal microbalance with dissipation (QCM-D) monitoring. The QCM-D estimates changes in the mass and viscous constant for the adsorbed layer through measurements of frequency and dissipation.Protein interaction with each of the test materials caused a net increase in mass of the material indicating protein binding except for lysozyme interacting with P(HEMA–MAA). A net decrease in mass was observed for lysozyme interacting with P(HEMA–MAA) which may be ascribed to lysozyme collapsing the hydrogel by expelling water. A net mass decrease was observed for cholesterol interacting with each of the hydrogel materials, while a mass increase was observed on PMMA.

Keywords
Hydrogels; Protein adsorption; Quartz crystal microbalance; Contact lens material
First Page Preview
The effect of charged groups on protein interactions with poly(HEMA) hydrogels
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 27, Issue 4, February 2006, Pages 567–575
Authors
, , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering