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Number-concentration of nanoparticles in liposomal and polymeric multiparticulate preparations: Empirical and calculation methods

Paper ID Volume ID Publish Year Pages File Format Full-Text
11994 771 2006 9 PDF Available
Title
Number-concentration of nanoparticles in liposomal and polymeric multiparticulate preparations: Empirical and calculation methods
Abstract

The actual number of particles in formulations of nanoparticles (NP) is of importance for quality assurance, comprehensive physicochemical characterization, and pharmacodynamics. Some calculation methods that have been previously employed are limited because they rely on several assumptions and are not applicable for certain preparations. Currently there are no validated experimental methods for determining the particle number-concentration (Nc) of liposomal and polymeric nanoparticulate preparations (<500 nm). This study examines a new empirical method for counting the number of particles in nanoparticulate formulations including drug-containing liposomes and polymeric NP. In the new method, suspended NP are nebulized to form aerosol droplets which are dried and counted using a scanning mobility particle sizer (SMPS). Experiments were conducted with three different preparations, empty liposomes (200 and 400 nm), drug-loaded liposomes (200 nm), and polymeric NP (150 nm). It was verified that no detrimental morphological or structural changes of the formulations have been induced by the SMPS technique, and that the obtained Nc values represent the original particles. It is concluded that nano-formulations with concentrations of up to 107 particles per 1 cm3 air, corresponding to approximately 1012 particles per 1 ml solution, can be directly counted within the size range of 30–900 nm. The measured values are compared to newly developed theoretical calculations to assess the viability of these calculations.

Keywords
Particles; Number concentration; Nanoparticles; Liposomes
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Number-concentration of nanoparticles in liposomal and polymeric multiparticulate preparations: Empirical and calculation methods
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 27, Issue 4, February 2006, Pages 651–659
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us