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Biomineralized porous composite scaffolds prepared by chemical synthesis for bone tissue regeneration

Paper ID Volume ID Publish Year Pages File Format Full-Text
1200 78 2010 10 PDF Available
Title
Biomineralized porous composite scaffolds prepared by chemical synthesis for bone tissue regeneration
Abstract

Scaffold design is a key factor in the clinical success of bone tissue engineering grafts. To date, no existing single biomaterial used in bone repair and regeneration fulfils all the requirements for an ideal bone graft. In this study hydroxyapatite/polycaprolactone (HA/PCL) composite scaffolds were prepared by a wet chemical method at room temperature. The physico-chemical properties of the composite materials were characterized by X-ray diffraction, Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy, while scaffold morphology was investigated by scanning electron microscopy (SEM) with energy-dispersive spectroscopy to validate the process used for synthesis. Finally, the response of bone marrow-derived human mesenchymal stem cells (hMSCs) in terms of cell proliferation and differentiation to the osteoblastic phenotype was evaluated using the Alamar blue assay, SEM and alkaline phosphatase activity. Microstructural analysis indicated that the HA particles were distributed homogeneously within the PCL matrix. The biological results revealed that the HA/PCL composite scaffolds are suitable for the proliferation and differentiation of MSCs in vitro, supporting osteogenesis after 15 days. All the results indicate that these scaffolds meet the requirements of materials for bone tissue engineering and could be used for many clinical applications in orthopaedic and maxillofacial surgery.

Keywords
Composite material; Hydroxyapatite; Scaffold; Human mesenchymal stem cell; Tissue engineering
First Page Preview
Biomineralized porous composite scaffolds prepared by chemical synthesis for bone tissue regeneration
Publisher
Database: Elsevier - ScienceDirect
Journal: Acta Biomaterialia - Volume 6, Issue 10, October 2010, Pages 4090–4099
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering