The inhibition of neutrophil antibacterial activity by ultra-high molecular weight polyethylene particles
Following infection, bacterial killing by polymorphonuclear leukocytes (neutrophils) is the main host defense against bacteria. Our hypothesis is that particles of ultra-high molecular weight polyethylene (UHMWP) may impair local neutrophil function and consequently reduce neutrophil bacterial killing. To determine how the in vitro phagocytic–bactericidal activity of neutrophils was affected by exposure to wear particles, tests were run comparing the effects of different particle composition, and different concentrations and sizes of UHMWP particles. There was a significant correlation between the number of particles and the decrease in neutrophil bactericidal activity (p<0.01p<0.01), and the greatest effect was obtained with a concentration of 107 UHMWP/ml. There was a significant decrease in neutrophil bactericidal activity by incubation with particles of 0.1–5 μm (p<0.01p<0.01), but not with larger size. The results suggest that neutrophil functional defects triggered by the presence of UHMWP particles may potentially contribute to the susceptibility of loose implants to bacterial infections.
Journal: Biomaterials - Volume 26, Issue 27, September 2005, Pages 5552–5557