fulltext.study @t Gmail

Characterization of a bovine collagen–hydroxyapatite composite scaffold for bone tissue engineering

Paper ID Volume ID Publish Year Pages File Format Full-Text
12026 773 2003 11 PDF Available
Title
Characterization of a bovine collagen–hydroxyapatite composite scaffold for bone tissue engineering
Abstract

Different biomaterials have been used as scaffolds for bone tissue engineering. Here we characterize a biomaterial composed of sintered (1100°C) and powdered hydroxyapatite (HA) and type I collagen (Coll), both of bovine origin, designed for osteoconductive and osteoinductive scaffolds. Coll/HA proportions were 1/2.6 and 1/1 (wet weight), and particles sizes varied from 200 to 400 μm. Vv (volume density) and Sv (surface to volume density) for the HA particles in the composite ranged from 0.48±0.06 to 0.55±0.02 and 5.090±0.545 to 6.366±0.289 μm−1, respectively. Due to the relatively small changes in Vv and Sv, a macroporosity could be characterized for the biocomposite. X-ray diffraction and infrared spectroscopy showed that the sintered bone was composed essentially of HA with minimum additional groups such as surface calcium hydroxide, surface and crystal water, free carbon dioxide and possibly brushite. Mass spectrometry detected carbonates at A and B sites of HA, and weakly bound to the structure. Human osteoblasts adhered and spread on both the HA particle surface and the collagen fibers, which seemed to guide cells between adjacent particles. The biocomposite studied has several characteristics considered as ideal for its use as a scaffold for osteoconduction and osteoinduction.

Keywords
Hydroxyapatite; Bovine bone; Characterization; Bone tissue engineering; Osteoblast; Collagen; Biocomposite; Biomaterial
First Page Preview
Characterization of a bovine collagen–hydroxyapatite composite scaffold for bone tissue engineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 24, Issue 27, December 2003, Pages 4987–4997
Authors
, , , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us