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Pulsatile perfusion and cardiomyocyte viability in a solid three-dimensional matrix

Paper ID Volume ID Publish Year Pages File Format Full-Text
12028 773 2003 6 PDF Available
Title
Pulsatile perfusion and cardiomyocyte viability in a solid three-dimensional matrix
Abstract

Background: The manufacture of full thickness three-dimensional myocardial grafts by means of tissue engineering is limited by the impeded cellular viability in unperfused in vitro systems. We introduce a novel concept of pulsatile tissue culture perfusion to promote ubiquitous cellular viability and metabolism.Methods: In a novel bioreactor we established pulsatile flow through the embedded three-dimensional tissue culture. Fibrin glue served as the ground matrix wherein neonatal rat cardiomyocytes were inoculated. Fluor-Deoxy-Glucose-Positron-Emission-Tomography (FDG-PET) and life/dead assays were employed for comparative studies of glucose uptake resp. cell viability.Results: A solid 8 mm thick structure resulted. Cellular viability significantly increased in the perfused chambers. We observed centripetal migration of the embedded cardiomyocytes to the site of the core vessel. However, cellular viability was high in the periphery of the tissue block too. FDG–PET revealed enhanced metabolic activity in perfused chambers.Conclusions: The present concept is highly effective in enhancing cellular viability and metabolism in a three-dimensional tissue culture environment. It could be utilized for various co-culture systems and the generation of viable tissue grafts.

Keywords
Cardiac tissue engineering; Angiogenesis; Collagen structure; Viability; Organ culture
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Pulsatile perfusion and cardiomyocyte viability in a solid three-dimensional matrix
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 24, Issue 27, December 2003, Pages 5009–5014
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
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Any Questions? feel free to contact us