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The significance of crystallographic texture of titanium alloy substrates on pre-osteoblast responses

Paper ID Volume ID Publish Year Pages File Format Full-Text
12039 775 2006 8 PDF Available
Title
The significance of crystallographic texture of titanium alloy substrates on pre-osteoblast responses
Abstract

The aim of this study is to investigate the effects of grain orientation in polycrystalline materials on cell-substrate interactions. Samples are prepared from rods and sheets of Ti–6Al–4V substrates with predominately two distinct crystallographic orientations. X-ray diffraction analysis indicates that 36% of the surfaces of rod samples consist of (101¯0) plane, while the predominant orientation in the surface of the sheet samples is (112¯0) plane (29%). Morphological studies and cell biological experiments including cell attachment, proliferation and differentiation are conducted using MC3T3 pre-osteoblast cells cultured on these two different samples. The number of attached cells on the rod Ti-(101¯0) samples (70% after 1 h and 50% after 2 h) is higher than on the sheet Ti-(112¯0) samples. Cell proliferation after 3 days is also significantly higher on the Ti-(101¯0) samples. Alkaline phosphatase activity, however, shows no significant difference between the two samples. Scanning electron microscopy (SEM) analysis of MC3T3 cells grown on samples with different crystallographic texture demonstrate significant differences in morphology with respect to attachment and growth pattern. This study shows that crystal orientation of the substrate can influence cell responses and, therefore, substrate engineering can be used to improve and control cell-substrate interactions.

Keywords
Titanium alloy; Crystallographic texture; Cell adhesion; Cell proliferation; Alkaline phosphatase; Cell morphology
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The significance of crystallographic texture of titanium alloy substrates on pre-osteoblast responses
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 27, Issue 19, July 2006, Pages 3532–3539
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us