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Protein layer coating method on metal surface by electrochemical process through genetical introduced tag

Paper ID Volume ID Publish Year Pages File Format Full-Text
12062 776 2005 4 PDF Available
Title
Protein layer coating method on metal surface by electrochemical process through genetical introduced tag
Abstract

The technology of mercaptide self-assembly has previously been used to coat proteins onto metals, but is not practical for many surfaces. In this study, a short peptidic tag was employed for the electrochemical immobilization of proteins on metals (ECtag). A 6-mer histidine (α-amino-1H-imidazole-4-propionic acid) homopeptide was employed as an ECtag ligand, which forms coordinate bonds with Ni2+ and other divalent metal ions. Protein A was chosen as a model protein for the immobilization, and was genetically tagged with an ECtag for immobilization onto a Pt electrode surface through the reduction of ECtag:Ni2+ to ECtag:Ni by the electrode potential.

Keywords
Protein immobilization; Biofilm; Surface modification; Biosensor; Electrode
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Protein layer coating method on metal surface by electrochemical process through genetical introduced tag
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 26, Issue 24, August 2005, Pages 4944–4947
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us