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Influence of carboxyl group density on neuron cell attachment and differentiation behavior: Gradient-guided neurite outgrowth

Paper ID Volume ID Publish Year Pages File Format Full-Text
12064 776 2005 8 PDF Available
Title
Influence of carboxyl group density on neuron cell attachment and differentiation behavior: Gradient-guided neurite outgrowth
Abstract

A UV pre-irradiation step followed by a UV grafting step was used to graft poly(acrylic acid) (PAA) on polymeric substrates. These substrates were then used to investigate the influence of carboxyl groups (–COOH) on cell behavior. Both the attachment and differentiation behaviors of C17.2 cells showed a –COOH group density-dependent response. In order to achieve an even distribution of cells on a –COOH gradient surface for neuron differentiation studies, an Ar plasma post-treatment was applied to the PAA-grafted surfaces. It greatly improved the cell adhesion properties with little damage to –COOH groups. This allows uniform distributions of seeded cells even on substrates with –COOH gradients. A linear or stepped –COOH gradient was found to be capable of serving as a repelling cue to guide the outgrowth of neurites from C17.2 cells. Up to 3.7 times more cells developed neurites growing down the –COOH gradient than growing up it.

Keywords
Carboxyl groups; Grafting; Gradient; C17.2 cells; Neurite outgrowth guidance
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 26, Issue 24, August 2005, Pages 4956–4963
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us