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Ultrastructural localization of laminin-5 (γ2γ2 chain) in the rat peri-implant oral mucosa around a titanium-dental implant by immuno-electron microscopy

Paper ID Volume ID Publish Year Pages File Format Full-Text
12082 777 2005 8 PDF Available
Title
Ultrastructural localization of laminin-5 (γ2γ2 chain) in the rat peri-implant oral mucosa around a titanium-dental implant by immuno-electron microscopy
Abstract

Laminin-5 (Ln-5) is an important molecule associated with epithelial cell adhesion and migration. In the gingiva around the tooth, Ln-5 localizes within basement membranes between the junctional epithelium (JE) and the tooth or connective tissue. Recently, we reported that in the oral mucosa around a dental implant, Ln-5 is expressed within the basement membranes at the implant-peri-implant epithelium (PIE) interface, and at the PIE–connective tissue interface. However, the ultrastructural localization of Ln-5 within or along the PIE has not yet been reported. Therefore, peri-implant oral mucosa was treated with anti-Ln-5 (γ2γ2 chain) antibody and examined using immuno-electron microscopy. Ln-5 was localized in the cells of the innermost-third layer and basal layer of the PIE. A 100-nm-wide Ln-5-positive internal basal lamina (basement membrane) and hemidesmosomes as adhesion structures were formed at the apical portion of the implant–PIE interface. However, at the upper-middle portion of the interface, these adhesion structures were not observed. Furthermore, at the PIE–connective tissue interface, the Ln-5-positive external basal lamina (basement membrane) and hemidesmosomes were partially deficient. Judging from these findings, we concluded that Ln-5 contributes to the attachment of the PIE to the titanium surface, and that PIE attached to titanium at the apical portion of the dental implant–PIE interface.

Keywords
Laminin-5; Peri-implant epithelium; Junctional epithelium; Internal and external basal laminae; Titanium dental implant; Immuno-electron microscopy
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Ultrastructural localization of laminin-5 (γ2γ2 chain) in the rat peri-implant oral mucosa around a titanium-dental implant by immuno-electron microscopy
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 26, Issue 32, November 2005, Pages 6280–6287
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us