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Collagen-coated polylactide microspheres as chondrocyte microcarriers

Paper ID Volume ID Publish Year Pages File Format Full-Text
12085 777 2005 9 PDF Available
Title
Collagen-coated polylactide microspheres as chondrocyte microcarriers
Abstract

Polylactide (PLA) microspheres were coated with collagen for cell culture and injectable cell carriers. Utilizing a method of emulsion-solvent evaporation, PLA microspheres with diameter ranging from 180 to 280 μm were prepared, followed with aminolysis in hexanediamine/n-propanol solution to introduce free amino groups on their surfaces. After the amino groups were transferred into aldehyde groups by a treatment of glutaraldehyde, collagen type I was covalently coupled via Schiff base formation between the aldehyde groups and the amino groups on collagen molecules. Meanwhile, physically entangled collagen molecules were retained following a grafting-coating protocol to yield microspheres coated with larger amount of collagen. Aminolysis resulted in weight loss of the microspheres following a linear relationship with the aminolysis time. The NH2 and collagen contents existed on the microsphere surface were quantitatively determined by ninhydrin and hydroproline (Hyp) analyses, respectively. Larger amount of collagen was immobilized on the microspheres with higher content of NH2. In vitro chondrocyte culture revealed that the cells could attach, proliferate and spread on these PLA microspheres, in particular on the ones having higher content of collagen. These results show that the collagen-coated PLA microspheres are promising candidate as cell microcarriers.

Keywords
Microspheres; Polylactide; Chondrocytes; Collagen; Cell microcarriers
First Page Preview
Collagen-coated polylactide microspheres as chondrocyte microcarriers
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 26, Issue 32, November 2005, Pages 6305–6313
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering