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Pattern stability under cell culture conditions—A comparative study of patterning methods based on PLL-g-PEG background passivation

Paper ID Volume ID Publish Year Pages File Format Full-Text
12099 778 2006 8 PDF Available
Title
Pattern stability under cell culture conditions—A comparative study of patterning methods based on PLL-g-PEG background passivation
Abstract

Despite the rapidly increasing number of publications on the fabrication and use of micro-patterns for cell studies, comparatively little is know about the long-term stability of such patterns under cell culture conditions. Here, we report on the long-term stability of cellular patterns created by three different patterning techniques: selective molecular assembly patterning, micro-contact printing and molecular assembly patterning by lift-off. We demonstrate that although all three techniques were combined with the same background passivation chemistry based on assembly of a PEG-graft copolymer, there are considerable differences in the long-term stability between the three different pattern types under cell culture conditions. Our results suggest that these differences are not cell-dependent but are due to different (substrate-dependent) interactions between the patterned substrate, the passivating molecule and the serum containing cellular medium.

Keywords
Selective molecular assembly patterning; SMAP; Microcontact printing; Molecular assembly patterning by lift-off; MAPL; Pattern degradation
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Pattern stability under cell culture conditions—A comparative study of patterning methods based on PLL-g-PEG background passivation
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 27, Issue 12, April 2006, Pages 2534–2541
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us