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Biochemical consequences of alginate encapsulation: A NMR study of insulin-secreting cells

Paper ID Volume ID Publish Year Pages File Format Full-Text
12104 778 2006 10 PDF Available
Title
Biochemical consequences of alginate encapsulation: A NMR study of insulin-secreting cells
Abstract

In this study we explore the biochemical consequences of alginate encapsulation on βTC3 cells. 13C NMR spectroscopy and isotopomer analysis were used to investigate the effects of encapsulation on several enzymatic processes associated with the TCA cycle. Our data show statistically significant differences in various enzymatic fluxes related to the TCA cycle and insulin secretion between monolayer and alginate-encapsulated cultures. The principal cause for these effects was the process of trypsinization. Embedding the trypsinized cells in alginate beads did not have a compounded effect on the enzymatic fluxes of entrapped cells. However, an additional small but statistically significant decrease in insulin secretion was measured in encapsulated cells. Finally, differences in either enzymatic fluxes or glucose consumption as a function of bead diameter were not observed. However, differences in T2, assessed by 1H NMR microimaging, were observed as a function of bead diameter, suggesting that smaller beads became more organized with time in culture, while larger beads displayed a looser organization.

Keywords
Alginate; Metabolism; 13C NMR; Isotopomer
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 27, Issue 12, April 2006, Pages 2577–2586
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
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