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Influence of nanoporous alumina membranes on long-term osteoblast response

Paper ID Volume ID Publish Year Pages File Format Full-Text
12134 781 2005 7 PDF Available
Title
Influence of nanoporous alumina membranes on long-term osteoblast response
Abstract

A major goal of bone tissue engineering is to design better scaffold configuration and materials to better control osteoblast behavior. Nanoporous architecture has been shown to significantly affect cellular response. In this work, nanoporous alumina membranes were fabricated by a two-step anodization method to investigate bone cell response. Osteoblasts were seeded on nanoporous alumina membranes to investigate both short-term adhesion and proliferation and long-term functionality and matrix production. Cell adhesion and proliferation were characterized using a standard MTT assay and cell counting. The total protein content was measured after cell lysis using the BCA assay. Matrix production was characterized in terms of surface concentrations of calcium and phosphorous, components of bone matrix, using X-ray photoelectron spectroscopy (XPS). The results from nanoporous alumina membranes were compared with those of amorphous alumina, aluminum, commercially available ANOPORETM and glass. Results indicate improved osteoblast adhesion and proliferation and increased matrix production after 4 weeks of study.

Keywords
Bone tissue engineering; Nanoporous alumina; Matrix production; Osteoblast adhesion
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Influence of nanoporous alumina membranes on long-term osteoblast response
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 26, Issue 22, August 2005, Pages 4516–4522
Authors
, , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us