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Cell adhesive PET membranes by surface grafting of RGD peptidomimetics

Paper ID Volume ID Publish Year Pages File Format Full-Text
12141 781 2005 12 PDF Available
Title
Cell adhesive PET membranes by surface grafting of RGD peptidomimetics
Abstract

A non-peptide mimic of the Arg-Gly Asp (RGD) active sequence of adhesive proteins (such as vitronectin) has been equipped with two different spacer-arms for surface anchorage. The covalent grafting on poly(ethylene terephthalate) (PET) membrane was realized via the activation of the hydroxyl polymer chain-ends by tosylation followed by nucleophilic substitution. The surface density of peptidomimetics was determined by X-ray photoelectron spectroscopy (XPS), on the basis of F/C atomic ratios since a fluorine tag was incorporated into the RGD-like compounds. The biological activity of soluble peptidomimetics was evaluated versus isolated human integrin αvβ3 (vitronectin receptor), and versus CaCo2 cells. Inhibition of cellular adhesion was observed after pre-incubation of CaCo2 cells with soluble peptidomimetics. On the other hand a significant promotion of cellular adhesion resulted from the surface grafting of peptidomimetics on the PET culture substrate. The best performance was obtained with the RGD-like integrin ligand bearing a triethylene glycol spacer-arm.

Keywords
RGD peptidomimetics; Poly(ethylene terephthalate) membrane (PET); In vitro cell culture; CaCo2 cells; Vitronectin; Polymer surface modification; XPS analysis; Biocompatibility
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Cell adhesive PET membranes by surface grafting of RGD peptidomimetics
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 26, Issue 22, August 2005, Pages 4576–4587
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us