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Binding specificity of α-bilirubin-imprinted poly(methacrylic acid-co-ethylene glycol dimethylacrylate) toward α-bilirubin

Paper ID Volume ID Publish Year Pages File Format Full-Text
12153 781 2005 9 PDF Available
Title
Binding specificity of α-bilirubin-imprinted poly(methacrylic acid-co-ethylene glycol dimethylacrylate) toward α-bilirubin
Abstract

α-Bilirubin is an important index to determine the liver's functions. Poly(methacrylic acid-co-ethylene glycol dimethylacrylate) (poly(MAA-co-EGDMA)) imprinted with α-bilirubin was proposed and shown to be able to bind α-bilirubin specifically. The extraction condition was also discussed. Polymers prepared by imprinting bilirubin in poly(MAA-co-EGDMA) and in poly(β-cyclodextrin-co-EGDMA) were compared. In this work, binding specificity of molecularly imprinted polymer (MIP) toward the target template, α-bilirubin, is discussed. Adsorption profile of α-bilirubin by bilirubin-imprinted poly(MAA-co-EGDMA) was measured as a function of time, from where the equilibrium could be determined. Two hours was determined to be the proper time for adsorption. Biliverdin as a rather similar analog compound of bilirubin was chosen for comparison of binding specificity in this study. Progesterone as well as testosterone was also chosen for study because they both co-exist in serum with bilirubin and might interfere with binding capacity of imprinted poly(MAA-co  -EGDMA) toward αα-bilirubin. Specificity of this polymer for bilirubin was thus confirmed by tasks carried out in mixture solutions comprised of compounds above. It is true that biliverdin contained in the binary mixture did affect the binding capacity of bilirubin. Nevertheless, polymer's binding specificity for bilirubin was essentially sufficient for recognition of α-bilirubin in the presence of other compounds. How MIP identified the target template molecule, α-bilirubin, is also elucidated.

Keywords
α-Bilirubin; Binding specificity; Imprinted poly(MAA-co-EGDMA); Biliverdin; Progesterone; Testosterone; Binary mixture; Recognition; MIP
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Binding specificity of α-bilirubin-imprinted poly(methacrylic acid-co-ethylene glycol dimethylacrylate) toward α-bilirubin
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 26, Issue 22, August 2005, Pages 4684–4692
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us