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A highly sensitive cell assay for validation of purification regimes of alginates

Paper ID Volume ID Publish Year Pages File Format Full-Text
12162 782 2003 12 PDF Available
Title
A highly sensitive cell assay for validation of purification regimes of alginates
Abstract

Among the hydrogels used for microencapsulation of cells and tissues, alginate has been and will continue to be one of the most important biomaterials. A mandatory requirement for clinical immunoisolated transplantations is the reproducible production of biocompatible alginate. As shown here for alginates extracted from freshly collected algal stipes, the current assays used for validation of the quality of the alginate are not sufficient to screen for impurities arising from spores of gram-positive bacteria (and related contaminants). To assess the quality of alginate, we have developed a cell assay based on the induction of apoptosis in Jurkat cells. This assay allows in combination with the “modified mixed lymphocyte” assay a rapid and sensitive screening for any fibrosis-inducing impurities in alginate samples (even during the purification regime) as demonstrated by transplantation experiments performed in parallel with BB rats (exhibiting an elevated macrophage activity).The results clearly demonstrate that the quality of the input algal material is of key relevance for the production of transplantation-grade alginate.

Keywords
Alginate; Apoptosis; Bacterial spores; Implantation; Fibrosis; Immune reaction
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A highly sensitive cell assay for validation of purification regimes of alginates
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 24, Issue 23, October 2003, Pages 4161–4172
Authors
, , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us