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Endothelial cell adhesion to the fibronectin CS5 domain in artificial extracellular matrix proteins

Paper ID Volume ID Publish Year Pages File Format Full-Text
12172 782 2003 8 PDF Available
Title
Endothelial cell adhesion to the fibronectin CS5 domain in artificial extracellular matrix proteins
Abstract

This study examines the spreading and adhesion of human umbilical vein endothelial cells (HUVEC) on artificial extracellular matrix (aECM) proteins containing sequences derived from elastin and fibronectin. Three aECM variants were studied: aECM 1 contains lysine residues periodically spaced within the protein sequence and three repeats of the CS5 domain of fibronectin, aECM 2 contains periodically spaced lysines and three repeats of a scrambled CS5 sequence, and aECM 3 contains lysines at the protein termini and five CS5 repeats. Comparative cell binding and peptide inhibition assays confirm that the tetrapeptide sequence REDV is responsible for HUVEC adhesion to aECM proteins that contain the CS5 domain. Furthermore, more than 60% of adherent HUVEC were retained on aECM 1 after exposure to physiologically relevant shear stresses (⩽100 dynes/cm2). Finally, the levels of thrombogenic markers (tissue plasminogen activator and plasminogen activator inhibitor–1) secreted by HUVEC monolayers on aECM 1 were found to be similar to those secreted by HUVEC monolayers cultured on fibronectin. These characteristics, along with the physical strength and elasticity of crosslinked films prepared from these materials, make aECM proteins promising candidates for application in small-diameter vascular grafts.

Keywords
Cell adhesion; Endothelial cell; Extracellular matrix; Protein design; Vascular graft
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Endothelial cell adhesion to the fibronectin CS5 domain in artificial extracellular matrix proteins
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 24, Issue 23, October 2003, Pages 4245–4252
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us