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The design of polymer microcarrier surfaces for enhanced cell growth

Paper ID Volume ID Publish Year Pages File Format Full-Text
12173 782 2003 12 PDF Available
Title
The design of polymer microcarrier surfaces for enhanced cell growth
Abstract

A variety of neutral and cationic polymers based on polyamino acids were prepared and investigated as microcarriers for cell attachment and growth. Among neutral polymer particles including the alkylated poly(γ-methyl l-glutamate) (PG) particles, in which the hydrophobicity changes as a function of the length of the alkyl groups, and hydroxy terminal PG particles, the PG particle with the longest alkyl chain (PG-C12) demonstrated the highest cell attachment rate and highest rate of cell growth. Moreover, the introduction of hydroxyl groups (PG-OH) led to a deterioration of cell growth. Cell growth on cationic particles having primary amino groups was drastically dependent upon the anion exchange capacity (AEC). A higher AEC for aminated PG microcarriers inhibited cell growth. In contrast, a higher AEC for cross-linked poly(ε-lysine) (PL) microcarriers facilitated cell growth. Cell growth on cationic particles clearly showed a good correlation with the pKa,app of the microcarriers, but not with their AEC. The particles with low and high pKa values possessed toxically acidic and basic pH microenvironments near the surface, respectively. These microenvironments had cytotoxic effects. On the other hand, no correlation between attachment rate constants and high cell growth was observed. The aminated particles, in which pKa were controlled at neutral pH, and PG-C12 produced obviously higher cell growth than did a commercially available microcarrier.

Keywords
Cell proliferation; Fibroblast; Hydrophilicity; Microsphere; Polyamino acid
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 24, Issue 23, October 2003, Pages 4253–4264
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
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Any Questions? feel free to contact us