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Construction and enzymatic degradation of multilayered poly-l-lysine/DNA films

Paper ID Volume ID Publish Year Pages File Format Full-Text
12204 784 2006 8 PDF Available
Title
Construction and enzymatic degradation of multilayered poly-l-lysine/DNA films
Abstract

The layer-by-layer (LbL) self-assembly of poly-l-lysine (PLL) and deoxyribonucleic acid (DNA) was used to construct the enzymatic biodegradable multilayered films. The LbL build up of DNA multilayers was monitored by UV–vis spectrometry, and atomic force microscopy (AFM). AFM, UV–vis spectrometry and fluorescence spectrometry measurements indicated that 90% of DNA within the films was released almost linearly under 5 U mL–1α–chymotrypsin in PBS at 37 °C in 35 h. TEM and zeta potential experiments revealed that the released DNA molecules were condensed into the slight positive complexes with size from 20 to several hundred nanometers. The well-structured, easy processed enzymatic biodegradable multilayered film may have great potential for gene applications in tissue engineering, medical implants, etc.

Keywords
Biodegradable; DNA delivery; Layer-by-layer; Poly-l-lysine; Self-assembly
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Construction and enzymatic degradation of multilayered poly-l-lysine/DNA films
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 27, Issue 7, March 2006, Pages 1152–1159
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us