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Combined use of designed scaffolds and adenoviral gene therapy for skeletal tissue engineering

Paper ID Volume ID Publish Year Pages File Format Full-Text
12205 784 2006 7 PDF Available
Title
Combined use of designed scaffolds and adenoviral gene therapy for skeletal tissue engineering
Abstract

While tissue engineering remains the most researched alternative to conventional therapies for repair and regeneration, how to optimally combine two of the most promising techniques, designed solid scaffolds and localized gene therapy, is largely unknown. We have conducted a systematic screening of several variables that may affect generation of bone via adenoviral gene therapy vector delivery, on image-based designed and solid freeform-fabricated scaffolds. These variables included: gene therapy type (ex vivo or in vivo); scaffold base material (sintered hydroxyapatite or a polypropylene fumarate/ tricalcium phosphate (PPF/TCP) composite), secondary carrier used to attach the biofactor to the scaffold (fibrin gel or a poly-lactic acid sponge), and scaffold pores size (300 or 800 μm). The in vivo formation of bone following implantation of these scaffolds was then analyzed. Gene therapy method had the largest effect, with ex vivo gene therapy yielding significant amounts of bone on nearly all the implants and in vivo gene therapy failing to produce any bone on most implants. Secondary carrier was the next most important variable, with fibrin gel consistently producing bone encompassing the implants and producing 2–4 times as much bone as the polymer sponge, which triggered only isolated bone growth. Though both scaffold base materials allowed bone growth, hydoxyapatite scaffolds generated twice as much bone as PPF/TCP scaffolds. The pore sizes tested had no significant effect on tissue generation.

Keywords
Adenovirus; Gene therapy; Hydroxyapatite; Bone; Free form fabrication; Poly-lactic acid
First Page Preview
Combined use of designed scaffolds and adenoviral gene therapy for skeletal tissue engineering
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 27, Issue 7, March 2006, Pages 1160–1166
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering