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Monocyte viability on titanium and copper coated titanium

Paper ID Volume ID Publish Year Pages File Format Full-Text
12211 785 2005 9 PDF Available
Title
Monocyte viability on titanium and copper coated titanium
Abstract

The role of apoptosis/cell death in the inflammatory response at the implanted materials is unexplored. Two surfaces with different cytotoxic potential and in vivo outcomes, titanium (Ti) and copper (Cu) were incubated in vitro with human monocytes and studied using a method to discriminate apoptotic and necrotic cells (Annexin V/PI staining). Further, staurosporine, a potent inducer of apoptosis, was added to the surface adherent monocytes. Lactate dehydrogenase (a marker of cell membrane injury) and TNF-α and IL-10, cytokines, previously suggested to play a major role in the monocyte apoptosis, were assayed in the culture medium.The results demonstrated that Ti surfaces displayed enhanced monocyte survival and production of IL-10 and TNF-α. Cu adherent cells exhibited apoptotic signs as early as 1 h after incubation. In contrast to Ti, after 48 h the predominance of apoptotic cells switched to apoptotic/necrotic cells on Cu surfaces. Staurosporine treatment of Ti adherent cells mediated similar type of cell death. LDH and cytokine contents were low around Cu surfaces, partly explained by interference between Cu ions and LDH and cytokines.This study suggests that material properties rapidly influence the onset of human monocyte apoptosis and progression to late apoptosis/necrosis. Early detection of apoptosis and cell death may be important for the understanding of the biological response to implanted materials.

Keywords
Titanium; Copper; Biocompatibility; Monocyte; Apoptosis; Cell viability; Cytokine
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Monocyte viability on titanium and copper coated titanium
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 26, Issue 30, October 2005, Pages 5942–5950
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us