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In vitro behavior of a porous TiO2/perlite composite and its surface modification with fibronectin

Paper ID Volume ID Publish Year Pages File Format Full-Text
12231 786 2005 14 PDF Available
Title
In vitro behavior of a porous TiO2/perlite composite and its surface modification with fibronectin
Abstract

In this study, we introduce a porous composite material, termed “Ecopore”, and describe in vitro investigation of the material and its modification with fibronectin. The material is a sintered compound of rutile TiO2 and the volcanic silicate perlite with a macrostructure of interconnecting pores. It is both inexpensive and easy to manufacture. We first investigated Ecopore for corrosion and leaching of elements in physiological saline. The corrosion supernatants did not contain critical concentrations of toxic trace elements. In an in vitro model, human primary osteoblasts (HOB) were cultured directly on Ecopore. HOB grew on the composite as well as on samples of its single constituents, TiO2 and perlite glass, and remained vital, but cellular spreading was less than on tissue culture plastic. The pro-inflammatory cytokines IL-1 and TNF-α were below detection limits in HOB culture supernatants, whereas IL-6 was detectable on a low level. To enhance cellular attachment and growth, the surface of the composite was modified by etching, functionalization with aminosilane and coupling of fibronectin. This modification greatly enhanced the spreading of HOB, indicated by vital staining and Sodium 3′-[1-(phenylaminocarbonyl)-3,4-tetrazolium]-bis (4-methoxy-6-nitro) benzene sulfonic acid hydrate (XTT) metabolism assays. HOB grew on the entire visible surface of porous fibronectin-modified composite, expressing alkaline phosphatase, a mature osteoblast marker. We conclude that Ecopore is non-toxic and sustains HOB growth, cellular spreading being improvable by coating with fibronectin. The composite may be usable in the field of bone substitution.

Keywords
Ceramic; Cytotoxicity; Surface modification; Fibronectin; OsteoblastBCA, bicinchoninic acid; DMEM, Dulbecco's modified Eagle's medium; EDS, energy dispersive spectroscopy; ELISA, enzyme-linked immunosorbent assay; FCS, fetal calf serum; HOB, Human osteobl
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In vitro behavior of a porous TiO2/perlite composite and its surface modification with fibronectin
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 26, Issue 16, June 2005, Pages 2813–2826
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us