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The recognition of adsorbed and denatured proteins of different topographies by β2 integrins and effects on leukocyte adhesion and activation

Paper ID Volume ID Publish Year Pages File Format Full-Text
12253 786 2005 15 PDF Available
Title
The recognition of adsorbed and denatured proteins of different topographies by β2 integrins and effects on leukocyte adhesion and activation
Abstract

Leukocyte β2 integrins Mac-1 and p150,95 are promiscuous cell-surface receptors that recognise and mediate cell adhesion to a variety of adsorbed and denatured proteins. We used albumin as a model protein to study whether leukocyte adhesion and activation depended on the nm-scale topography of a protein adlayer. Albumin adsorbed from the native conformation gave rise to different adlayer topographies and different amounts of adsorbed protein on hydrophobic and relatively hydrophilic polystyrene and silanised silicon-wafer surfaces, whereas adsorption of pre-denatured Alb resulted in similar adlayer topographies and similar amounts of adsorbed protein on these surfaces. All three distinct protein-adlayer topographies supported adhesion of in vitro differentiated, macrophage-like U937 and THP-1 cells, but did not support adhesion of their promonocytic precursors. Human monocytes freshly isolated from peripheral blood did not adhere to adsorbed albumin, not even in the presence of monocyte chemoattractant protein-1 and macrophage inflammatory protein-1α chemokines. Adhesion of the macrophage-like cells to albumin in any of the three topographies was inhibited by antibodies against β2 integrins, but not by antibodies against β1 integrins, and did not induce secretion of the proinflammatory cytokine tumour necrosis factor-α.

Keywords
Albumin; Integrin; Macrophage; Monocyte; Nanotopography; Protein adsorptionAFM, atomic force microscopy; AHS, N-(6-aminohexyl) aminopropyltrimethoxysilane; Alb, albumin; DPBS, Dulbecco's phosphate-buffered saline; dTHP-1, PMA-differentiated THP-1 cells; d
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The recognition of adsorbed and denatured proteins of different topographies by β2 integrins and effects on leukocyte adhesion and activation
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 26, Issue 16, June 2005, Pages 3039–3053
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us