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Microgrooved fibrillar collagen membranes as scaffolds for cell support and alignment

Paper ID Volume ID Publish Year Pages File Format Full-Text
12262 786 2005 10 PDF Available
Title
Microgrooved fibrillar collagen membranes as scaffolds for cell support and alignment
Abstract

For several years, microgrooved substrates have been evaluated as a means to orient cells in engineered tissues. Recently, we fabricated thin (0.1–5.3 μm) planar and tubular collagen membranes (CMs) from air-dried hydrogels of native, fibrillar type I collagen (Vernon et al., Biomaterials 2004;26:1109–17). The CMs were strong, stable, and permeable and, hence, of potential use as scaffolds for tissue engineering. In the present study, planar CMs supported a robust attachment, spreading, and proliferation of human dermal fibroblasts (HDFs) and human umbilical artery smooth muscle cells (HUASMCs). Collagen hydrogels were air-dried onto microgrooved templates and subsequently removed in the form of grooved CMs with the potential to align cells. The grooved CMs were highly effective at inducing HDFs and HUASMCs to elongate and align, as revealed by scanning electron microscopy and by assays of f-actin and nuclear orientation. Alignment of cells was maintained at high cell densities. CMs with grooves of substantially different widths and depths were similarly effective in causing cell alignment; however, cells aligned poorly on CMs that had grooves less than 1 μm in depth. Grooved CMs with the capability to align cells might be of considerable use in the fabrication of tissue substitutes.

Keywords
Cell culture; Collagen; Fibroblast; Hydrogel; Membrane; Microgroove; Micropatterning; Scaffold; Smooth muscle cell; Surface topography
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Microgrooved fibrillar collagen membranes as scaffolds for cell support and alignment
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 26, Issue 16, June 2005, Pages 3131–3140
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us