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Effect of an avidin–biotin binding system on chondrocyte adhesion, growth and gene expression

Paper ID Volume ID Publish Year Pages File Format Full-Text
12263 786 2005 11 PDF Available
Title
Effect of an avidin–biotin binding system on chondrocyte adhesion, growth and gene expression
Abstract

Cell adhesion to synthetic biomaterials is a prerequisite for anchorage cell culture and tissue engineering. The current study investigated utilization of an avidin–biotin binding system in enhancing chondrocyte adhesion to tissue culture polystyrene (TCPS). Biotinylated chondrocytes adhered to avidin-coated TCPS more quickly than untreated chondrocytes to bare TCPS. Also the avidin–biotin binding system enhanced cell initial spreading. However, the effects were only transient. The growth of biotinylated chondrocytes was first decreased during the first 3 days but increased afterwards. The progeny of biotinylated chondrocytes still maintained the ability in expressing cartilage extracellular matrix proteins such as type II collagen, type IX collagen and aggrecan. These results show potential for the application of the avidin–biotin binding system to cell culture and tissue engineering.

Keywords
Biotin; Avidin; Chondrocytes; Cell adhesion; Cell proliferation; Gene expression
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Effect of an avidin–biotin binding system on chondrocyte adhesion, growth and gene expression
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 26, Issue 16, June 2005, Pages 3141–3151
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us