fulltext.study @t Gmail

Characterization of the Flexcell™ Uniflex™ cyclic strain culture system with U937 macrophage-like cells

Paper ID Volume ID Publish Year Pages File Format Full-Text
12298 788 2006 8 PDF Available
Title
Characterization of the Flexcell™ Uniflex™ cyclic strain culture system with U937 macrophage-like cells
Abstract

Mechanical forces alter many cell functions in a variety of cell types. It has been recognized that stimulation of cells in culture may be more representative of some physiologic conditions. Although there are commercially available systems for the study of cells cultured in a mechanical environment, very little has been documented on the validation techniques for these devices. In this study, Flexcell's™ recently introduced Uniflex™ cyclic strain system was programmed to apply 10% longitudinal sinusoidal strain (0.25 Hz) for 48 h to U937 cells cultured on Uniflex™ plates. Image analysis was employed to characterize the actual strain field. For a chosen amplitude of 10% the applied strain was highly reproducible and relatively uniform (10.6±0.2%) in a central rectangular region of the membrane (dimensions of 9.2±2×13.6±0.8 mm2). The strain increased the release of IL-6, esterase and acid phosphatase activity (p<0.05) from adherent U937 cells. Cells also displayed altered morphology, aligning and lengthening with the direction of strain, whereas static cells maintained a round appearance showing no preferred orientation. These data indicate that cyclic mechanical strain applied by the Uniflex™ strain system modulates U937 cell function leading to selective increases in enzymatic activities as well as orientation in a favored direction.

Keywords
Macrophage; Mechanical test; Esterase; Uniaxial strain; Siloxane
First Page Preview
Characterization of the Flexcell™ Uniflex™ cyclic strain culture system with U937 macrophage-like cells
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 27, Issue 2, January 2006, Pages 226–233
Authors
, , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us