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The dependence of fibrillar adhesions in human fibroblasts on substratum chemistry

Paper ID Volume ID Publish Year Pages File Format Full-Text
12299 788 2006 12 PDF Available
Title
The dependence of fibrillar adhesions in human fibroblasts on substratum chemistry
Abstract

Little is known about the influence of substratum properties and composition on the ability of cells to translocate α5β1 integrins and to form fibrillar adhesion. We have examined the impact of self-assembled monolayers (SAMs) bearing different functional end groups (amines (NH2) or carboxylic acids (COOH)) on the presence of fibrillar adhesions in human fibroblasts attached to fibronectin-coated SAMs. Most of the fibroblasts incubated in serum-free medium for 2 h on COOH showed segregation of focal contact components (αv integrin subunits, phosphotyrosine proteins) and fibrillar adhesions (α5 integrin subunits, tensin) while the majority of cells plated on NH2 did not. Analysis of fibronectin fibril formation confirmed also that human fibroblasts plated on COOH formed matrix fibrils significantly better. The surface-associated α5 to αv integrin ratio was smaller in cells on COOH than on NH2 due to a decreased α5 integrin binding. In addition, human fibroblasts migrated more readily on COOH in comparison to NH2 which points to a different binding strength of integrins to the substratum. Overall, the results indicate that the molecular composition of substrata has a strong influence on FN matrix formation by promoting or inhibiting segregation of focal and fibrillar adhesions.

Keywords
Focal adhesions; Fibrillar adhesions; Integrin; Fibronectin; Cell migration
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The dependence of fibrillar adhesions in human fibroblasts on substratum chemistry
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 27, Issue 2, January 2006, Pages 234–245
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
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Any Questions? feel free to contact us