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The effect on osteoblast function of colocalized RGD and PHSRN epitopes on PEG surfaces

Paper ID Volume ID Publish Year Pages File Format Full-Text
12319 789 2005 12 PDF Available
Title
The effect on osteoblast function of colocalized RGD and PHSRN epitopes on PEG surfaces
Abstract

Poly(ethylene glycol) hydrogels were synthesized with pendant peptide functionalities to examine the influence of synergistic peptide sequences on osteoblast adhesion, spreading, and function. Specifically, acrylated monomers were prepared that contained the peptide sequence, Arg–Gly Asp (RGD), as well as monomers with RGD plus its synergy site, Pro–His–Ser–Arg–Asn (PHSRN), linked via a polyglycine sequence to recapitulate the native spacing of fibronectin. The colocalized RGD–PHSRN sequence improved osteoblast adhesion, spreading, and focal contact formation when compared to RGD alone. In addition, proliferation, metabolic activity, and levels of alkaline phosphatase production, a common marker for osteoblast function, were statistically higher for the colocalized peptide sequences at 1 day, 1 week, and 2 weeks, when compared to control surfaces. Interestingly, increases were not observed in all areas of cell function, as extracellular matrix (ECM) production was the lowest on gels functionalized with the colocalized peptide sequence. This result was attributed to strong receptor–ligand interactions initiating signal transduction cascades that down-regulate ECM production.

Keywords
Hydrogels; Poly(ethylene) glycol; RGD peptide; Extracellular matrix; Bone tissue engineering
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The effect on osteoblast function of colocalized RGD and PHSRN epitopes on PEG surfaces
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 26, Issue 25, September 2005, Pages 5209–5220
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us