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Prevention of the epoxy resin-based root canal sealers-induced cyclooxygenase-2 expression and cytotoxicity of human osteoblastic cells by various antioxidants

Paper ID Volume ID Publish Year Pages File Format Full-Text
12377 791 2005 7 PDF Available
Title
Prevention of the epoxy resin-based root canal sealers-induced cyclooxygenase-2 expression and cytotoxicity of human osteoblastic cells by various antioxidants
Abstract

Cyclooxygenase-2 (COX-2) is an inducible enzyme believed to be responsible for prostaglandin synthesis at the site of inflammation. Recently, the activation of COX-2 expression may be one of the important pathogenesis of root canal sealers-induced periapical inflammation. However, little is known about whether chemical interaction can modulate the epoxy resin-based root canal sealers-induced cytotoxicity as well as COX-2 expression. The aim of the present study was to investigate the effects of antioxidants catalase, superoxide dismutase (SOD), and N-acetyl-L-cysteine (NAC) on AH26- and Topseal-induced COX-2 mRNA gene and cytotoxicity in human osteoblastic cell line U2OS cells. The results showed that both epoxy resin-based root canal sealers were cytotoxic to U2OS cells in a concentration-dependent manner (p<0.05). AH26 and Topseal were found to induce COX-2 mRNA gene expression in U2OS cells. The addition of glutathione (GSH) precursor NAC led to decrease the induction of COX-2 mRNA gene expression and cytotoxicity by both AH26 and Topseal (p<0.05). However, catalase and SOD lacked the ability to prevent AH26-and Topseal-induced cytotoxicity and COX-2 mRNA gene expression (p>0.05). Taken together, the activation of COX-2 mRNA gene expression may be one of the pathogenesis of epoxy resin-based root canal sealers-induced periapical inflammation. In addition, GSH depletion, but not the attack of oxygen free radicals, could be the mechanism for epoxy resin-based root canal sealers-induced cytotoxicity and COX-2 mRNA gene expression. Factors that induce GSH synthesis may appear useful in preventing cell damage mediated by epoxy resin-based root canal sealers.

Keywords
Root canal sealers; Epoxy resin; Cyclooxygenase-2; Glutathione; Antioxidants
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Prevention of the epoxy resin-based root canal sealers-induced cyclooxygenase-2 expression and cytotoxicity of human osteoblastic cells by various antioxidants
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 26, Issue 14, May 2005, Pages 1849–1855
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us