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DNA encapsulated magnesium and manganous phosphate nanoparticles: potential non-viral vectors for gene delivery

Paper ID Volume ID Publish Year Pages File Format Full-Text
12409 791 2005 7 PDF Available
Title
DNA encapsulated magnesium and manganous phosphate nanoparticles: potential non-viral vectors for gene delivery
Abstract

Nanoparticles of Mg and Mn (II) phosphates encapsulating pDNA were prepared. The sizes of these DNA loaded particles in aqueous dispersion were about 100–130 nm diameter, and they aggregated with the progression of time. Although magnesium phosphate nanoparticles were crystalline, the manganous phosphate nanoparticles were found to be amorphous in nature. Nanoparticle dissolution and pDNA release were studied using atomic absorption spectroscopy and gel electrophoresis experiments. These inorganic phosphate nanoparticles dissolved in mild acidic pH (∼5) releasing pDNA indicating that DNA release in the endosomal compartment is possible. In vitro transfection in HeLa cells demonstrated that while magnesium phosphate nanoparticles showed 100% efficiency, manganous phosphate nanoparticles exhibited about 85% transfection efficiency compared to that of ‘polyfect’, as control.

Keywords
Magnesium phosphate; Manganous phosphate; Nanoparticles; Non-viral vectors; Gene delivery; Transfection efficiency
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DNA encapsulated magnesium and manganous phosphate nanoparticles: potential non-viral vectors for gene delivery
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 26, Issue 14, May 2005, Pages 2157–2163
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us