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Multi-channel 3-D cell culture device integrated on a silicon chip for anticancer drug sensitivity test

Paper ID Volume ID Publish Year Pages File Format Full-Text
12410 791 2005 8 PDF Available
Title
Multi-channel 3-D cell culture device integrated on a silicon chip for anticancer drug sensitivity test
Abstract

A novel three-dimensional cell culture system was constructed with an array of cell panels (4×5) in a silicon chip, together with multi-channel drug containers. Human breast cancer (MCF-7) cells were embedded in a collagen–gel matrix and entrapped in a pyramidal-shaped silicon hole. Each cell panel can be isolated by a channel composed of a microfluid part and a reservoir. A cell panel was exposed to 200 mm KCN for 2 days to demonstrate that each cell panel could be independently evaluated under various stimulation conditions. Based on the cellular respiration activity, the proliferation behavior was continuously monitored on the silicon-based cell array for 5 days using scanning electrochemical microscopy (SECM). The cells entrapped in the device (3-D culture) proliferated normally, and the proliferation rate was lower than that of cells grown in a monolayer cell culture (2-D culture). The effects of three anticancer drugs measured simultaneously on the cell chip were in good agreement with those obtained by a conventional colorimetric assay. Our results suggest that the silicon-based device for 3D culture is appropriate for a chemosensitivity assay involving multi-chemical stimulation.

Keywords
Biosensor; Collagen; Cytotoxicity; Electrochemistry; Polydimethylsiloxane
First Page Preview
Multi-channel 3-D cell culture device integrated on a silicon chip for anticancer drug sensitivity test
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 26, Issue 14, May 2005, Pages 2165–2172
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering