fulltext.study @t Gmail

Combinatorial screening of cell proliferation on poly(l-lactic acid)/poly(d,l-lactic acid) blends ☆

Paper ID Volume ID Publish Year Pages File Format Full-Text
12448 793 2005 10 PDF Available
Title
Combinatorial screening of cell proliferation on poly(l-lactic acid)/poly(d,l-lactic acid) blends ☆
Abstract

We have combined automated fluorescence microscopy with a combinatorial approach for creating polymer blend gradients to yield a rapid screening method for characterizing cell proliferation on polymer blends. A gradient in polymer blend composition of poly(l-lactic acid) (PLLA) and poly(d,l-lactic acid) (PDLLA) was created in the form of a strip-shaped film and was annealed to allow PLLA to crystallize. Fourier transform infrared (FTIR) microspectroscopy was used to determine the composition in the gradients and atomic force microscopy was used to characterize surface topography. Osteoblasts were cultured on the gradients and proliferation was assessed by automated counting of cells using fluorescence microscopy. Surface roughness varied with composition, was smooth on PDLLA-rich regions and was rough on the PLLA-rich regions. Cell adhesion was similar on all regions of the gradients while proliferation was faster on the smooth, PDLLA-rich end of the gradients than on the rough, PLLA-rich end of the gradients. These results demonstrate the feasibility of a new, combinatorial approach for evaluating cell proliferation on polymer blends.

Keywords
Combinatorial methods; Polymer blends; Cell proliferation; Polylactic acid; Microspectroscopic FTIR; Osteoblast
First Page Preview
Combinatorial screening of cell proliferation on poly(l-lactic acid)/poly(d,l-lactic acid) blends ☆
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 26, Issue 34, December 2005, Pages 6906–6915
Authors
, , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us