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The use of poly(ethylene oxide) for the efficient stabilization of entrapped α-chymotrypsin in silicone elastomers: A chemometric study

Paper ID Volume ID Publish Year Pages File Format Full-Text
12455 793 2005 11 PDF Available
Title
The use of poly(ethylene oxide) for the efficient stabilization of entrapped α-chymotrypsin in silicone elastomers: A chemometric study
Abstract

The enzyme α-chymotrypsin, a model for catalytic proteins, was entrapped in different silicone elastomers that were formed via the condensation-cure room temperature vulcanization (CC-RTV) of silanol terminated poly(dimethylsiloxane) with tetraethyl orthosilicate as a crosslinker, in the presence of different poly(ethylene oxide) oligomers that were functionalized with triethoxysilyl groups. The effects of various chemical factors on both the activity and entrapping efficiency of proteins (leaching) were studied using a 2-level fractional factorial design—a chemometrics approach. The factors studied include the concentration and chain length of poly(ethylene oxide), enzyme content, and crosslinker (TEOS) concentration. The study indicated that poly(ethylene oxide) can stabilize the entrapped α-chymotrypsin in silicone rubber: the specific activity can be maximized by incorporating a relatively high content of short chain, functional PEO. Increased enzyme concentration was found to adversely affect the specific activity. The effect of TEOS was found to be insignificant when PEO was present in the elastomer, however, it does affect the activity positively in the case of simple elastomers.

Keywords
Immobilized enzyme; α-chymotrypsin; Silicone elastomer; Poly(ethylene oxide); Chemometrics
First Page Preview
The use of poly(ethylene oxide) for the efficient stabilization of entrapped α-chymotrypsin in silicone elastomers: A chemometric study
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 26, Issue 34, December 2005, Pages 6973–6983
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering