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Substrate chemistry influences the morphology and biological function of adsorbed extracellular matrix assemblies

Paper ID Volume ID Publish Year Pages File Format Full-Text
12477 793 2005 15 PDF Available
Title
Substrate chemistry influences the morphology and biological function of adsorbed extracellular matrix assemblies
Abstract

In addition to mediating cell signalling events, native extracellular matrix (ECM) assemblies interact with other ECM components, act as reservoirs for soluble signalling molecules and perform structural roles. The potential of native ECM assemblies in the manufacture of biomimetic materials has not been fully exploited due, in part, to the effects of substrate interactions on their morphology. We have previously demonstrated that the ECM components, fibrillin and type VI collagen microfibrils, exhibit substrate dependent morphologies on chemically and topographically variable heterogeneous surfaces. Using both cleaning and coating approaches on silicon wafers and glass coverslips we have produced chemically homogeneous, topographically similar substrates which cover a large amphiphilic range. Extremes of substrate amphiphilicity induced morphological changes in periodicity, curvature and lateral spreading which may mask binding sites or disrupt domain structure. Biological functionality, as assayed by the ability to support cell spreading, was significantly reduced for fibrillin microfibrils adsorbed on highly hydrophilic substrates (contact angle 20.7°) compared with less hydrophilic (contact angle 38.3°) and hydrophobic (contact angle 92.8°) substrates. With an appropriate choice of surface chemistry, multifunctional ECM assemblies retain their native morphology and biological functionality.

Keywords
Extracellular matrix (ECM); Atomic force microscopy (AFM); Surface energy; Surface roughness; Protein adsorption; Cell spreading
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Substrate chemistry influences the morphology and biological function of adsorbed extracellular matrix assemblies
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 26, Issue 34, December 2005, Pages 7192–7206
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
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Any Questions? feel free to contact us