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Photomediated crosslinking of C6-cinnamate derivatized type I collagen

Paper ID Volume ID Publish Year Pages File Format Full-Text
12505 794 2005 9 PDF Available
Title
Photomediated crosslinking of C6-cinnamate derivatized type I collagen
Abstract

Synthesis and characterization of cinnamated Type I collagen and its related mechanical properties after photomediated crosslinking were investigated in detail. Using an EDC/NHS conjugation method, collagen was chemically modified to incorporate a photosensitive cinnamate moiety. The cinnamated collagen was fully characterized by 1H NMR, UV–vis, and circular dichroism (CD) spectroscopy, as well as by rheological and mechanical analyses. Cinnamated collagens with varying degrees of derivatization retained collagen triple helical structure. The rheological spectra of collagen solutions demonstrate that the storage modulus decreases with increasing cinnamate content, owing to a decrease in physical crosslinking. The kinetics of the crosslinking process in both hydrated gels and dry films were monitored by UV–vis spectroscopy and confirmed that crosslinking was complete within 60 min of irradiation. The uniaxial stress–strain behavior of crosslinked collagen films, including Young's modulus and ultimate tensile strength, was comparable to values reported for glutaraldehyde-crosslinked monomeric collagen films. These data demonstrate that derivatization of collagen with photosensitive cinnamate moieties provides a facile route for solid-state crosslinking, thereby improving the mechanical properties of collagen and enhancing the potential applicability of collagen-based materials in tissue engineering and drug delivery.

Keywords
Collagen; Crosslinking; Photocrosslinkable; Photodimerizable; Cinnamate; Blood vessel
First Page Preview
Photomediated crosslinking of C6-cinnamate derivatized type I collagen
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 26, Issue 18, June 2005, Pages 4041–4049
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering