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Bioactive sol–gel glasses with and without a hydroxycarbonate apatite layer as substrates for osteoblast cell adhesion and proliferation

Paper ID Volume ID Publish Year Pages File Format Full-Text
12509 795 2003 11 PDF Available
Title
Bioactive sol–gel glasses with and without a hydroxycarbonate apatite layer as substrates for osteoblast cell adhesion and proliferation
Abstract

The biocompatibility of three sol–gel bioactive glasses with SiO2 as the main constituent (75, 72.5 and 70 mol%), identical CaO content (25 mol%), and without or with P2O5 as third constituent (0, 2.5 and 5 mol%), have been analyzed (S75, S72.5P2.5, and S70P5 glasses). These studies were performed on both untreated glasses and on glasses coated with a hydroxycarbonate apatite (HCA) layer formed in vitro by soaking 7 d in an acellular simulated body fluid. Cell attachment, spreading and proliferation were studied using neonatal rat calvaria osteoblasts. Cells attach to the three untreated glasses but show a higher efficiency on that with the higher phosphate content (S70P5). The formation of the HCA layer significantly enhances this process (1.7-fold). In all cases, attachment is followed by cell spreading on the surface of the materials, adopting the cells a flattened morphology and showing diverse anchoring cell projections. Mitotic activity has been detected on osteoblasts growing on the sol–gel glasses, being this process 2–4-fold higher when the apatite-like layer is already formed. Taking into account the results herein presented, these bioactive glasses can be considered biocompatible. In addition, their biocompatibility is greatly enhanced after induction of the formation of an HCA layer.

Keywords
Bioactive glasses; Sol–gel; Mineralization; Biocompatibility; Osteoblast culture
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Bioactive sol–gel glasses with and without a hydroxycarbonate apatite layer as substrates for osteoblast cell adhesion and proliferation
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 24, Issue 20, September 2003, Pages 3383–3393
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us