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Osteoblasts response to allogenic and xenogenic solvent dehydrated cancellous bone in vitro

Paper ID Volume ID Publish Year Pages File Format Full-Text
12513 795 2003 10 PDF Available
Title
Osteoblasts response to allogenic and xenogenic solvent dehydrated cancellous bone in vitro
Abstract

The present in vitro study investigates the cellular interaction of primary human osteoblasts with human and bovine solvent dehydrated cancellous bone (SDCB) discs. These are bio-implants from solvent dehydrated, gamma-irradiated preserved human and bovine cancellous bone, pre-treated to remove all cells, genetic components and water soluble proteins. Primary human osteoblasts were harvested from cancellous chips of trauma patients undergoing osteosynthesis with bone grafting from the iliac crest. All patients provided informed consent. The present investigation tested proliferation, synthesis of phenotypic marker, and morphology of primary cultured human osteoblasts on SDCB in vitro. The total protein and collagen type 1 content could not be revealed, due to the inherent naturally occurring protein content in these two bio-implants. In conclusion, our in vitro results suggest that SDCB may be a suitable bone substitute which provides a well structured and biocompatible scaffold for ingrowing human osteoblasts.

Keywords
Human osteoblasts; SDCB; Osteocalcin; Alkaline phosphatase; RT-PCR; Immunocytochemistry; Morphology
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Osteoblasts response to allogenic and xenogenic solvent dehydrated cancellous bone in vitro
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 24, Issue 20, September 2003, Pages 3417–3426
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us