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Characterization and performance of membranes designed for macroencapsulation/implantation of pancreatic islet cells ☆

Paper ID Volume ID Publish Year Pages File Format Full-Text
12520 795 2003 9 PDF Available
Title
Characterization and performance of membranes designed for macroencapsulation/implantation of pancreatic islet cells ☆
Abstract

Amphiphilic polymer membranes were synthesized for macroencapsulation of cells and characterized by select chemical and biological techniques. The membranes were prepared by crosslinking hydrophilic poly(N,N-dimethyl acrylamide) (PDMAAm) main chains with hydrophobic di-, tri-, and octa-methacrylate telechelic polyisobutylene (PIB) stars. The hydrophilic/hydrophobic composition and the molecular weights between crosslink sites (both Mc,hydrophilic and Mc,hydrophobic) were controlled by synthesis conditions. Small tubular membranes were made by in situ rotational copolymerization/crosslinking and filled with pancreatic rat islets. The water-swelling behavior, mechanical properties, and oxygen and insulin diffusion were studied. Macroencapsulatory performance of these membranes was investigated in vitro by macroencapsulation of pancreatic rat islets within tubular membranes for up to 1.5 months, and studying the insulin secreting ability of encapsulated islets in culture. The membranes are robust and maintain their integrity for the period of encapsulation. They allow oxygen and insulin diffusion. Macroencapsulated islets maintained their viability and insulin secretion over an extended period (i.e., 45 days).

Keywords
Amphiphilic polymer membranes; Macroencapsulation of pancreatic islets; Macroencapsulatory membranes; Oxygen permeability; Glucose challenge
First Page Preview
Characterization and performance of membranes designed for macroencapsulation/implantation of pancreatic islet cells ☆
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 24, Issue 20, September 2003, Pages 3483–3491
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering