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Morphological characterization and in vitro biocompatibility of a porous nickel–titanium alloy

Paper ID Volume ID Publish Year Pages File Format Full-Text
12543 799 2005 7 PDF Available
Title
Morphological characterization and in vitro biocompatibility of a porous nickel–titanium alloy
Abstract

Disks consisting of macroporous nickel–titanium alloy (NiTi, Nitinol®, Actipore®) are used as implants in clinical surgery, e.g. for fixation of spinal dysfunctions. The morphological properties were studied by scanning electron microscopy (SEM) and by synchrotron radiation-based microtomography (SRμCT). The composition was studied by X-ray diffractometry (XRD), differential scanning calorimetry (DSC), and energy-dispersive X-ray spectroscopy (EDX). The mechanical properties were studied with temperature-dependent dynamical mechanical analysis (DMA). Studies on the biocompatibility were performed by co-incubation of porous NiTi samples with isolated peripheral blood leukocyte fractions (polymorphonuclear neutrophil granulocytes, PMN; peripheral blood mononuclear leukocytes, PBMC) in comparison with control cultures without NiTi samples. The cell adherence to the NiTi surface was analyzed by fluorescence microscopy and scanning electron microscopy. The activation of adherent leukocytes was analyzed by measurement of the released cytokines using enzyme-linked immunosorbent assay (ELISA). The cytokine response of PMN (analyzed by the release of IL-1ra and IL-8) was not significantly different between cell cultures with or without NiTi. There was a significant increase in the release of IL-1ra (p<0.001p<0.001), IL-6 (p<0.05p<0.05), and IL-8 (p<0.05p<0.05) from PBMC in the presence of NiTi samples. In contrast, the release of TNF-α by PBMC was not significantly elevated in the presence of NiTi. IL-2 was released from PBMC only in the range of the lower detection limit in all cell cultures. The material, clearly macroporous with an interconnecting porosity, consists of NiTi (martensite; monoclinic, and austenite; cubic) with small impurities of NiTi2 and possibly NiCx. The material is not superelastic upon manual compression and shows a good biocompatibility.

Keywords
Shape memory alloys; Biocompatibility; Cytokines; Leukocytes; Porosity; Nickel–titanium alloys
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Morphological characterization and in vitro biocompatibility of a porous nickel–titanium alloy
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 26, Issue 29, October 2005, Pages 5801–5807
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us