fulltext.study @t Gmail

Selective targeting of antibody-conjugated nanoparticles to leukemic cells and primary T-lymphocytes

Paper ID Volume ID Publish Year Pages File Format Full-Text
12554 799 2005 9 PDF Available
Title
Selective targeting of antibody-conjugated nanoparticles to leukemic cells and primary T-lymphocytes
Abstract

In the present study, surface-modified nanoparticles based on biodegradable material were used for antibody coupling in order to get a selective drug carrier systems. Gelatin nanoparticles were prepared by a desolvation process. Sulfhydryl groups were introduced which enabled the linkage of NeutrAvidin (NAv). Antibodies specific for the CD3 antigen on lymphocytic cells were conjugated to the nanoparticles surface. The binding of biotinylated anti-CD3 antibody was achieved by NAv–biotin-complex formation. Cellular binding and uptake were determined by flow cytometry and confocal laser scanning microscopy (CLSM). Cell-type-specific targeting of anti-CD3-conjugated nanoparticles into CD3-positive human T-cell leukemia cells and primary T-lymphocytes could be shown. Celluar uptake and effective internalization of antibody-conjugated nanoparticles into CD3 expressing cells were demonstrated. Uptake rates of about 84% into T-cell leukemia cells were observed. To confirm selectivity of T-cell targeting, competition experiments were carried out adding excessive free anti-CD3 prior to nanoparticle incubation leading to significantly reduced cellular uptake of antibody-conjugated nanoparticles. Further analysis on the mechanism of uptake confirmed a receptor-mediated endocytotic process. Protein-based nanoparticles conjugated with an antibody against a specific cellular antigen hold promise as selective drug delivery systems for specific cell types.

Keywords
Nanoparticles; Drug targeting; T-lymphocytic leukemia cells; T-lymphocytes; Cellular uptake; AntibodiesαCD3-NP, anti-CD3-conjugated gelatin nanoparticles; CLSM, confocal laser scanning microscopy; Nav-FITC, fluorescein isothiocyanate-conjugated NeutrAvidi
First Page Preview
Selective targeting of antibody-conjugated nanoparticles to leukemic cells and primary T-lymphocytes
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 26, Issue 29, October 2005, Pages 5898–5906
Authors
, , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us