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Biotransformation and liver-specific functions of human hepatocytes in culture on RGD-immobilized plasma-processed membranes

Paper ID Volume ID Publish Year Pages File Format Full-Text
12622 805 2005 10 PDF Available
Title
Biotransformation and liver-specific functions of human hepatocytes in culture on RGD-immobilized plasma-processed membranes
Abstract

In this paper we report on the metabolic response of human hepatocytes grown on polyethersulfone membranes surface modified with a plasma-deposited acrylic acid coating and RGD peptide covalently immobilized through a “spacer arm” molecule. The modified surfaces were characterized by means of X-ray photoelectron spectroscopy and water contact angle measurements.The performance of modified and unmodified membranes was evaluated by assessing the expression of liver specific and biotransformation functions of human hepatocytes. Diclofenac, a non-steroidal anti-inflammatory drug, was used to investigate the biotransformation functions.Surface-modified membranes elicit specific cellular responses and induce hepatocytes to enhance the synthesis rate of albumin and urea, particularly in the presence of diclofenac. Also the biotransformation functions were expressed at high levels.

Keywords
Membrane; Plasma modification; RGD immobilization; Human hepatocytes; Liver-specific functions; Drug biotransformation
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Biotransformation and liver-specific functions of human hepatocytes in culture on RGD-immobilized plasma-processed membranes
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 26, Issue 21, July 2005, Pages 4432–4441
Authors
, , , , , , , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us