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Soluble phosphate glasses: in vitro studies using human cells of hard and soft tissue origin

Paper ID Volume ID Publish Year Pages File Format Full-Text
12636 806 2004 10 PDF Available
Title
Soluble phosphate glasses: in vitro studies using human cells of hard and soft tissue origin
Abstract

This report describes the short-term response of two typical cellular components of a hard/soft tissue interface such as the periodontal ligament/mandible and patellar tendon/tibia. Tissue engineering of such interfaces requires a contiguous scaffold system with at least two cell types associated with it. Human oral osteoblasts, oral fibroblasts and hand flexor tendon fibroblasts were seeded on phosphate-based glasses of different dissolution rates. Quantitative and morphological assessment of cell adhesion and proliferation for all cell types was assessed, after first elucidating an experimental composition range using MG63 cells. In addition, immunolabelling of bone-specific non-collagenous proteins bone sialoprotein, osteonectin and osteopontin was performed to determine osteoblast phenotype. Fibroblast phenotype was established by immunolabelling for prolyl-4-hydroxylase, an enzyme vital for collagen biosynthesis. Results indicated that both cell types maintained their respective phenotypes over time in culture on glass discs of generic composition (CaO)x–(Na2O)0.5−x–(P2O5)0.5, remained attached and proliferated dependent on glass composition and cell type. Glasses containing at least 46 mol% CaO, produced no adverse cell reaction suggesting that these compositions that support both osteoblasts and fibroblasts would be ideal as a scaffold material for engineering the hard/soft tissue interface.

Keywords
Glass; Bone tissue engineering; Ligament; Osteoblast; Fibroblast
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Soluble phosphate glasses: in vitro studies using human cells of hard and soft tissue origin
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 25, Issue 12, May 2004, Pages 2283–2292
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us