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Differentiation, growth and activity of rat bone marrow stromal cells on resorbable poly(l/dl-lactide) membranes

Paper ID Volume ID Publish Year Pages File Format Full-Text
12638 806 2004 9 PDF Available
Title
Differentiation, growth and activity of rat bone marrow stromal cells on resorbable poly(l/dl-lactide) membranes
Abstract

Nonporous and porous membranes produced from poly(l/dl-lactide) 80/20% were characterized using profilometry, contact-angle measurements, infra-red spectroscopy, X-ray photoemission spectroscopy and scanning electron microscopy, and used to culture bone marrow stromal cells isolated from the rat femora. The cells were cultured for 5, 10, 15 and 20 days. Cell growth and activity was estimated from the amounts of DNA, alkaline phosphatase activity and total protein amount present in the cell lysate and cell differentiation was assessed histochemically. Cell morphology was estimated from scanning electron microscopy. The cells fully expressed osteoblastic phenotype, revealed spindle-shaped, ellipsoidal morphology, developed podia, produced an abundant fibrillar extracellular matrix and mineral noduli. The number of cells on the membranes increased with time of culturing and was higher for the porous membranes than the nonporous membranes. Osteoblastic differentiation was most significant between 5 and 10 days of culture. The total amounts of DNA, alkaline phosphatase and proteins increased with time of culturing. The surface characteristics of the porous membranes were superior to the nonporous membranes.

Keywords
Bone marrow stromal cells; Polylactide membrane; Tissue engineering; Cell culture; Osteoblast differentiation
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Differentiation, growth and activity of rat bone marrow stromal cells on resorbable poly(l/dl-lactide) membranes
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 25, Issue 12, May 2004, Pages 2299–2307
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us