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Protein adsorption, attachment, growth and activity of primary rat osteoblasts on polylactide membranes with defined surface characteristics

Paper ID Volume ID Publish Year Pages File Format Full-Text
12642 806 2004 11 PDF Available
Title
Protein adsorption, attachment, growth and activity of primary rat osteoblasts on polylactide membranes with defined surface characteristics
Abstract

The adsorption of proteins and growth and activity of primary rat osteoblasts cultured for 1, 2 and 3 weeks on nonporous and porous resorbable poly(l/dl-lactide) 80/20% membranes with defined surface characteristics were investigated. The growth and activity of cells were estimated from the measurements of DNA, alkaline phosphatase activity and the total amount of protein in the cell lysate. The cell morphology was assessed from scanning electron microscopy and rhodamine staining. The protein adsorption to the membrane surface was assessed from the amide I peak at 1640–1660 cm−1 and the amide II peak at 1540–1560 cm−1 in the attenuated total reflection infrared spectra. The relative amount of proteins adsorbed on the nonporous and porous membranes was comparable. The cells growing on the nonporous and porous membranes maintained the phenotype and revealed morphology typical for osteoblasts. The mineralized noduli were larger in size on the porous membranes. The number of cells, the amount of DNA, the alkaline phosphatase activity, and the total amount of protein increased with time of the experiment and were higher for the porous membranes than for the nonporous ones.

Keywords
Osteoblasts; Polylactide; Resorbable membranes; Cell culture; Protein adsorption
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Protein adsorption, attachment, growth and activity of primary rat osteoblasts on polylactide membranes with defined surface characteristics
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 25, Issue 12, May 2004, Pages 2341–2351
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us