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Mineralization behaviour of collagen type I immobilized on different substrates

Paper ID Volume ID Publish Year Pages File Format Full-Text
12645 806 2004 10 PDF Available
Title
Mineralization behaviour of collagen type I immobilized on different substrates
Abstract

Collagen type I as a robust fibre protein and main component of the extracellular matrix of most tissues is increasingly utilized for surface engineering of biomaterials using different immobilization methods. In the present work we studied the mineralization behaviour of fibrillar collagen type I in simulated body fluid as a measure for conformational changes caused by adsorptive immobilization or immobilization by partial incorporation into the anodic oxide layer on c.p.-titanium using microscopic and vibration spectroscopic methods. Adsorptive immobilization on highly oriented pyrolytic graphite (HOPG) and c.p.-titanium without collagen were used as references.In the initial phase (1–24 h) the kinetics of formation and the morphology of calcium phosphate phases (CPP) are strongly influenced both by the substrate and the immobilization method. Compared to HOPG both types of immobilization on titanium increasingly inhibit the formation of CPP. For longer times (30 d) these initial differences disappear-mineralization product on titanium, irrespective of the presence of collagen, is a mixture of amorphous calcium phosphate and octacalcium phosphate. Contrary to this the mineralization of HOPG substrates results in hydroxy apatite. This is discussed with respect to the conditions during the immobilization as well as the resulting interactions between substrate and immobilized collagen. It is shown that the mineralization process exhibits a high sensitivity with respect to conformational changes caused by these interactions. Possible cell biological relevance of these conformational changes is discussed.

Keywords
Biomineralization; Calcium phosphate; Collagen structure; FTIR; SBF (Simulated body fluid); Titanium
First Page Preview
Mineralization behaviour of collagen type I immobilized on different substrates
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 25, Issue 12, May 2004, Pages 2371–2380
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering