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Fabrication of a protein film by electrospray deposition method and investigation of photochemical properties by persistent spectral hole burning

Paper ID Volume ID Publish Year Pages File Format Full-Text
12660 808 2003 7 PDF Available
Title
Fabrication of a protein film by electrospray deposition method and investigation of photochemical properties by persistent spectral hole burning
Abstract

Transparent protein film of iron-free cytochrome c (Cyt. c) was successfully manufactured by electrospray deposition (ES) method and the properties of the protein film were investigated. Excursion temperature dependence of spectral hole profiles in the photochemical hole burning for the iron-free Cyt. c in protein film and in glassy solution was investigated and compared with that of iron-free porphyrin embedded in a synthetic polymer film to clarify photochemical properties of electrospray deposited protein film. The spectral holes of iron-free porphyrin were thermally more stable in Cyt. c than in polymer, indicating compact packing of the chromophore in the protein. The ES-deposited iron-free Cyt. c in protein film showed less stable spectral hole than that in glassy solution. This difference is attributable to the electrospray deposition process of the protein involving ionization and to subsequent cross-linking of protein molecules.

Keywords
Electrospray deposition; Protein film; Photochemical hole burning (PHB); Iron-free cyt. c 
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Fabrication of a protein film by electrospray deposition method and investigation of photochemical properties by persistent spectral hole burning
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 24, Issue 12, May 2003, Pages 2045–2051
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering