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In vitro biocompatibility assessment of poly(ε-caprolactone) films using L929 mouse fibroblasts

Paper ID Volume ID Publish Year Pages File Format Full-Text
12674 809 2004 9 PDF Available
Title
In vitro biocompatibility assessment of poly(ε-caprolactone) films using L929 mouse fibroblasts
Abstract

Biodegradable and biocompatible materials are the basis for tissue engineering. As an initial step for developing vascular grafts, the in vitro biocompatibility of poly(ε-caprolactone) (PCL), recently suggested for several clinical applications, was evaluated in this study using L929 mouse fibroblasts. Different cellular aspects were analyzed in order to know the cell viability during cell culture on PCL films: adhesion, proliferation, morphology, LDH release and mitochondrial function. Since topography and other surface characteristics of materials play an essential part in cell adhesion, PCL membranes with either smooth or rough surface were prepared, characterized and used to carry out cell cultures. During short culture times, PCL produced a significant stimulation of mitochondrial activity evaluated by reduction of the MTT reagent. The results provide evidences of good adhesion, growth, viability, morphology and mitochondrial activity of cells on PCL films. Therefore, it can be concluded that PCL is a suitable and biocompatible material as a scaffold for vascular graft development.

Keywords
Polycaprolactone; Biocompatibility; Cell adhesion; Cell proliferation; MTT assay; Fibroblast
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In vitro biocompatibility assessment of poly(ε-caprolactone) films using L929 mouse fibroblasts
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 25, Issue 25, November 2004, Pages 5603–5611
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us