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Preparation of collagen/calcium phosphate multilayer sheet using enzymatic mineralization

Paper ID Volume ID Publish Year Pages File Format Full-Text
12704 813 2004 9 PDF Available
Title
Preparation of collagen/calcium phosphate multilayer sheet using enzymatic mineralization
Abstract

The multilayer sheets (2–10 layers), which consisted of alternately cumulated collagen and calcium phosphate layers with the thickness of 6–8 μm in each layer, were prepared. The inorganic layer was mineralized by means of an alkaline phosphatase-catalyzed hydrolysis of water-soluble phosphate esters in the presence of calcium ions. The calcium phosphate, which was formed on the collagen, was assayed as a mixture of hydroxyapatite (main) and amorphous calcium phosphate. The multilayer sheets were not only strong mechanically but also semitransparent and flexible in a dry state. Furthermore, the collagen/calcium phosphate multilayer sheets did not swell in water to keep the original morphology. As a scaffold, the sheets having the calcium phosphate layer on the top supported the attachment and growth of L929 fibroblast cells. The enzymatic mineralization and the collagen/calcium phosphate composite sheets were discussed in conjunction with physicochemical and biological properties.

Keywords
Alkaline phosphatase; Calcium phosphate; Collagen; Fibroblast; Hydroxyapatite
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Preparation of collagen/calcium phosphate multilayer sheet using enzymatic mineralization
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 25, Issue 24, November 2004, Pages 5481–5489
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
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Full-text PDF Download
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